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Image Search Results
Journal: JCI Insight
Article Title: Allograft dendritic cell p40 homodimers activate donor-reactive memory CD8 + T cells
doi: 10.1172/jci.insight.96940
Figure Lengend Snippet: Groups of C57BL/6 (n = 4/group) mice received A/J cardiac allografts subjected to 8 hours of CIS. On days 0 and 1 after transplant, all recipients were injected with 100 μg BrdU i.p. and either 250 μg control rat IgG or (A) 250 μg CTLA-4Ig or (B) 250 μg anti-CD154 mAb. After 48 hours, allografts were harvested and digested, and aliquots of single cell suspensions were stained with antibody and analyzed by flow cytometry, with examples of gating as shown for each allograft sample to assess and quantitate the BrdU incorporation of infiltrating memory CD4+ and CD8+ T cells.***P < 0.001, as determined by the Mann-Whitney nonparametric test. (C) Groups of C57BL/6 recipient mice (n = 5–6/group) received A/J cardiac allografts subjected to 8 hours of CIS and, on days 0 and 1 after transplant, were treated with 250 μg control rat IgG, 250 μg CTLA-4Ig, or 250 μg anti-CD154 mAb i.p. Allograft survival was monitored by daily abdominal palpation, and rejection was confirmed by laparotomy. **P < 0.01 versus 8 hours of CIS + IgG or + CTLA-4Ig, as determined using the Log-rank/Mantel-Cox test.
Article Snippet:
Techniques: Injection, Staining, Flow Cytometry, BrdU Incorporation Assay, MANN-WHITNEY
Journal: Cell
Article Title: Single-cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment
doi: 10.1016/j.cell.2018.05.060
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: SEQC is available on https://github.com/ambrosejcarr/seqc.git and Biscuit is available on https://github.com/sandhya212/BISCUIT_SingleCell_IMM_ICML_2016 . table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Biological Samples Breast carcinoma Fresh operative tissue samples at MSKCC N/A Normal breast tissue Fresh operative tissue samples at MSKCC N/A Lymph node Fresh operative tissue samples at MSKCC N/A Peripheral blood Patient venipuncture at MSKCC N/A Antibodies anti-CD45 ab Biolegend RRID:AB_2566372 DAPI Stain Calbiochem SCR_014366 anti-Foxp3 ab Thermo Fisher RRID:AB_1834364 anti-CD3 ab Biolegend RRID:AB_1575008 anti-CD4 ab Biolegend RRID:AB_571945 anti-CD16 ab Biolegend RRID:AB_314207 anti-CD56 ab BD Biosciences RRID:AB_396853 anti-CD8 ab Biolegend RRID:AB_528885 anti-CD19 ab Biolegend RRID:AB_2562015 anti-CD11b ab Biolegend RRID:AB_2563395 anti-CD45 Y89 Fluidigm RRID:AB_2261851 anti-CD235ab BioLegend RRID:AB_314620 anti-CD61 BioLegend RRID:AB_1227584 anti-CD196/CCR6 Pr141 Fluidigm Cat:3141014A anti-CD19 142Nd Fluidigm RRID:AB_2651155 anti-CD278/ICOS 143Nd Fluidigm Cat:3143025B anti-CD270 144Nd Fluidigm Cat:3144022B anti-CD4 145Nd Fluidigm RRID:AB_2661789 anti-CD8a 146Nd Fluidigm Cat:3146001B anti-CD11c 147Sm Fluidigm RRID:AB_2687850 anti-CD14 148Nd Fluidigm Cat:3148010B anti-CD127 149Sm Fluidigm RRID:AB_2661792 anti-TIGIT eBioscience Cat:16-9500-82 anti-CD123 151Eu Fluidigm RRID:AB_2661794 anti-CD95/Fas 152Sm Fluidigm Cat:3152017B anti-CD45RA 153Eu Fluidigm Cat:3153001B anti-TIM3 154Sm Fluidigm Cat:3154010B anti-CD39 BioLegend RRID:AB_940438 anti-CD274/PD-L1 156Gd Fluidigm Cat: 3156026B anti-CD27 158Gd Fluidigm Cat: 3158010B anti-CD357/GITR 159Tb Fluidigm Cat: 3159020B anti-CD28 160Gd Fluidigm Cat: 3160003B anti-CD152/CTLA4 161Dy Fluidigm Cat: 3161004B anti-FoxP3 162Dy Fluidigm RRID:AB_2687650 anti-CD33 163Dy Fluidigm RRID:AB_2687857 anti-CD45RO 164Dy Fluidigm Cat: 3164007B anti-CD223/LAG3 165Ho Fluidigm RRID:AB_2687859 anti-Ki67 BD RRID:AB_396287 anti-CD197/CCR7 167Er Fluidigm Cat:3167009A anti-CD154/CD40L 168Er Fluidigm Cat:
Techniques: Staining, Antibody Labeling, Software, Diffusion-based Assay